Application of Plant Extracts in Micropropagation and Cryopreservation of Bleeding Heart: An Ornamental-Medicinal Plant Species

Lamprocapnos spectabilis (L.) Fukuhara (bleeding heart) is valued both in the horticultural and pharmaceutical markets. Despite its great popularity, information on vitro tissue culture technology this species limited. There also little knowledge application of plant extracts systems plants other than orchids. The aim study to compare utility traditional growth regulators (PGRs) natural extracts—obtained from coconut shreds, as well oat, rice, sesame seeds—in micropropagation cryopreservation L. ‘Gold Heart’ ‘White Gold’. biochemical analysis composition included. In first experiment related via axillary buds activation, single-node explants were cultured for a 10-week-long propagation cycle modified Murashige Skoog medium fortified either with 1.11 µM benzyladenine (BA) 1.23 indole-3-butritic acid (IBA) or 10% (v/v) extracts. A PGRs- extract-free control was considered. experiment, same added into during seven-day preculture encapsulation-vitrification protocol. It found that impact additives cultivar- trait-specific. addition extract favoured proliferation shoots ratio bleeding heart Heart’. Rice extract, hand, promoted callus formation Gold’ cultivar more effective increasing conventional (4.1 2.6, respectively). Sesame suppressed development cultivars analysed, probably due high content polyphenols. As second did not increase survival level cryopreserved shoot tips (sesame oat even decreased parameter). On abundant simple sugars endogenous cytokinins, stimulated intensive after rewarming samples. Analysing synergistic effect should be considered future studies spectabilis.